Keeb Kwm Tshawb Fawb thiab Qhov Tseem Ceeb
Hauv kev tsim tshuaj tiv thaiv kab mob FMD, cov kab mob hauv cov kab mob muaj cov khib nyiab ntau heev. Yog tias raug lim ncaj qha rau 0.2 μm sterilizing, cov ntaub lim dej yuav ua rau muaj kev qias neeg sai, txo qhov ua haujlwm thiab muaj peev xwm cuam tshuam rau qhov permeability thiab cov txiaj ntsig ntawm lub hom phiaj antigen (146S viral particles).
Txoj kev tshawb fawb no tsom mus rau peb lo lus nug tseem ceeb:
Piv txwv qhov kev ua tau zoo ntawm kev lim dej saum npoo av thiab kev lim dej tob hauv cov nqe lus ntawm kev ua haujlwm, kev txo qhov turbidity, thiab kev tiv thaiv ntawm cov lim dej sterilizing downstream.
Txheeb xyuas qhov zoo tshwj xeeb ntawm cov lim dej tob uas muaj zog zoo hauv kev tshem tawm cov khib nyiab ntawm tes.
Tsim kom muaj cov txheej txheem lim dej uas nthuav dav tau rau cov kua dej cog qoob loo uas muaj ntau qib turbidity sib txawv, kom ua tau raws li cov kev xav tau ntawm kev tsim khoom hauv kev lag luam.
Cov Kev Sim Tseem Ceeb thiab Cov Txiaj Ntsig
Kev Lim Dej Nto vs. Kev Lim Dej Qhov Tob
Kev sim:Cov kua tshuaj tiv thaiv FMD uas tsis ua haujlwm tau lim los ntawm kev siv daim nyias nyias polypropylene 0.45 μm thiab cov ntawv lim tob, ua raws li kev lim dej sterilizing 0.2 μm.
Cov Kev Tshawb Pom:
Qhov pib turbidity: 77 NTU.
Kev lim dej tob:throughput 641 L/m²; txo qhov turbidity mus rau 9.65 NTU (ntshiab thiab kaj).
Kev lim dej saum npoo:tsuas yog 46 L/m² xwb; qhov turbidity tseem yog 41 NTU.
Kev lim dej sterilizing downstream:Tom qab kev lim dej tob, cov lim dej 0.2 μm muaj qhov ntws tawm ntawm 655 L / m², piv rau tsuas yog 91 L / m² tom qab kev lim dej saum npoo av - qhov kev txhim kho 7.2 ×.
146S cov txiaj ntsig:Kev lim dej tob dua li kev lim dej saum npoo av.
Xaus lus:Qhov kev lim dej tob tob yog 13.9 × siab dua li kev lim dej saum npoo av, qhov turbidity ntawm kev lim dej qis dua 76.5%, thiab lub neej lim dej sterilizing tau ntev dua 7 ×. Los ntawm ob qho tib si tus nqi thiab cov txiaj ntsig, kev lim dej tob tob zoo dua.
Kev lim dej tob uas muaj zog thiab muaj zog
Kev sim:Ob hom ntawv lim dej tob uas muaj qhov loj tib yam tab sis qhov ceev ntawm cov nqi sib txawv (qhov zoo ib txwm piv rau qhov zoo muaj zog) tau raug sim rau cov kua dej FMD uas tsis ua haujlwm.
Cov Kev Tshawb Pom:
Cov qauv ntawm kev ua haujlwm thiab kev siab zoo sib xws.
Qhov tob zoo li qublim dej:qhov lim dej turbidity 25 NTU.
Qhov tob zoo heevlim dej:qhov lim dej turbidity 9.65 NTU (tsuas yog 38.6% ntawm qhov ib txwm).
Lub peev xwm them tau zoo los ntawm cov resin ligands tshwj xeeb uas txhim kho electrostatic adsorption.
Cov ntaub ntawv sau tseg qhia tias cov lim dej tob tob zoo kuj tseem tuaj yeem tshem tawm cov protein ntawm cov hlwb (HCP) thiab DNA.
Xaus lus:Cov lim dej tob zoo muaj zog ua rau txo qhov turbidity zoo dua (<10 NTU), tiv thaiv kev lim dej ultrafiltration/sterilizing downstream, thiab tshem tawm cov khoom tsis huv ntxiv uas cuam tshuam nrog cov txheej txheem.
1. Cov Ntsiab Cai thiab Cov Txiaj Ntsig ntawm Kev Lim Dej tob
Kev lim dej saum npoo:Tsuas yog ntes cov khoom me me ntawm qhov chaw ntawm daim nyias nyias xwb; feem ntau yog siv sieving raws li qhov loj me.
Kev lim dej tob:Tshem tawm cov khoom me me thoob plaws hauv lub matrix los ntawm kev ntes thiab electrostatic adsorption.
Cov txiaj ntsig tseem ceeb:
Tsis muaj asbestos (tsis zoo li cov lim dej thaum ub).
Ua los ntawm cov cellulose fibers uas muaj purity siab, cov khoom siv lim dej, thiab cov polymeric resins.
Cov pab pawg ua haujlwm nrog cov nqi zoo muab cov adsorption muaj zog rau cov khoom me me thiab cov khoom tsis huv.
Ua raws li cov cai tswjfwm biopharmaceutical tag nrho.
2. Cov Qauv Lim Uas Muaj Peev Xwm Ntsuas Tau Rau Cov Qib Turbidity Sib Txawv
Raws li kev tshawb fawb sib piv:
Qisqhov tsis meejcov kua dej:sib dua ua ntej lim dej txaus.
Nruab Nrabqhov tsis meejcov kua dej:pom zoo kom siv ob txheej lim dej tob.
Siabqhov tsis meejcov kua dej:Yuav tsum tau lim dej tob tob kom txo qhov turbidity qis dua 10 NTU, kom ntseeg tau tias kev ua haujlwm downstream zoo thiab muaj cov antigen ntau.
3. Kev Siv Khoom: Great Wall Filtration Depth Filters
Great Wall Filtration muaj cov txheej txheem lim dej tob uas tsim los rau cov txheej txheem biopharmaceutical.
Cov yam ntxwv tseem ceeb:
Cov ntawv lim dej tob muaj peev xwm siab rau kev tshem tawm cov cell, cov khib nyiab, thiab cov khoom loj.
Siv tau rau cov kab lis kev cai ntawm cov tsiaj txhu, cov kab mob bacterial / yeast lysates.
Muaj peev xwm tshem tawm HCP, DNA, cov kab mob, endotoxins, thiab cov neeg sawv cev tiv thaiv kev ua npuas dej.
Kev tsim qauv me me, scalable linearly, haum rau kev siv hauv-kab lossis sib luag.
Cov nqi ntws ruaj khov, lub neej ua haujlwm ntev, thiab tus nqi ua haujlwm qis.
Xaus lus
Cov thev naus laus zis lim dej tob uas muaj zog zoo ua rau kev ua kom pom tseeb thiab rov qab tau cov tshuaj antigen hauv kev tsim tshuaj tiv thaiv FMD zoo dua. Dhau li ntawm cov tshuaj tiv thaiv FMD, nws muab txoj hauv kev txhim khu kev qha rau kev ntxuav hauv lwm yam tshuaj lom neeg. Thaum kev ua kom zoo dua ntawm cov txheej txheem bioprocess txuas ntxiv mus, cov thev naus laus zis lim dej siab heev yuav tseem ceeb rau kev tsim tshuaj tiv thaiv kom muaj kev nyab xeeb thiab ua haujlwm tau zoo.
Tsab xov xwm no yog raws li cov ntaub ntawv sim tiag tiag rau kev siv xwb.Kev lees paubnyob rau hauv cov xwm txheej tiag tiag ntawm kev tsim khoom raug pom zoo.
